9: J Physiol Pharmacol. 2002 Dec;53(4 Pt 2):751-9.  
Effects of thapsigargin on stimulation frequency--dependent changes in mitochondrial calcium in rat cardiac myocytes.

Lewartowski BP.

Department of Clinical Physiology, Medical Center of Postgraduate Education, Marymoncka 99, 01-813, Warsaw, Poland.

We investigated in the single myocytes of rat heart the effect of blocking of ATP-ase of sarcoplasmic reticulum (SR) on mitochondrial Ca2+ uptake and release. Mitochondrial Ca2+ content was investigated as Mn(2+) - resistant fluorescence of Indo 1 - AM loaded into cells. SR ATP-ase was blocked with 10(-6) M thapsigargin (Tg). Tg blocked almost completely stimulation -dependent mitochondrial Ca2+ uptake and slowed down its release despite that the maximal cytosolic Ca2+ concentration was not decreased. We propose that mitochondrial stimulation -dependent Ca2+ uptake is greatly enhanced by [Ca2+] built by SR in microdomains adjacent to these organelle.

PMID: 12510861 [PubMed - indexed for MEDLINE]




[top] This page was done by a student of Bioinformatics, Open University Jerusalem Israel, in a homage to Fermentek and to Dr Berend.
 4mg  noneto biocompare formulae omd ToxicPlants DictCellBio MolBioDict ( test  position )
BioChemical Suppliers
A23187 Actinomycin Aflatoxin Anisomycin Aphidicolin Ascomycin


Cerulenin Chromomycin Geldanamycin Cyclopiazonic acid
Cytochalasin Forskolin Fumagillin Fumonisin Hypericin K252a
KT5823 Mitomycin Nigericin Ochratoxin Oligomycin Mycophenolic acid
Paclitaxel Patulin Paxilline Penitrem Puromycin Penicillic acid
Radicicol Rapamycin Staurosporine Sterigmatocystin Thapsigargin Tunicamycin
 Verruculogen  Wortmannin