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| 9: J Physiol Pharmacol. 2002 Dec;53(4 Pt 2):751-9. |
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- Effects of thapsigargin on stimulation
frequency--dependent changes in mitochondrial calcium in rat cardiac myocytes.
Lewartowski BP.
Department of Clinical Physiology, Medical Center of Postgraduate Education,
Marymoncka 99, 01-813, Warsaw, Poland. bohdanp@cmkp.edu.pl
We investigated in the single myocytes of rat heart the effect of blocking of
ATP-ase of sarcoplasmic reticulum (SR) on mitochondrial Ca2+ uptake and
release. Mitochondrial Ca2+ content was investigated as Mn(2+) - resistant
fluorescence of Indo 1 - AM loaded into cells. SR ATP-ase was blocked with
10(-6) M thapsigargin (Tg). Tg blocked almost completely stimulation
-dependent mitochondrial Ca2+ uptake and slowed down its release despite that
the maximal cytosolic Ca2+ concentration was not decreased. We propose that
mitochondrial stimulation -dependent Ca2+ uptake is greatly enhanced by [Ca2+]
built by SR in microdomains adjacent to these organelle.
PMID: 12510861 [PubMed - indexed for MEDLINE]
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