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| 6: Cancer Lett. 2003 Feb 28;191(1):83-92. |
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- The effect of ursodeoxycholic acid on the survivin in
thapsigargin-induced apoptosis.
Sohn J, Khaoustov VI, Xie Q, Chung CC, Krishnan B, Yoffe B.
Department of Medicine, Baylor College of Medicine and Veterans Affairs
Medical Center (151B), 2002 Holcombe Boulevard, Houston, TX 77030, USA.
Endoplasmic reticulum (ER) was recently suggested as a third subcellular
compartment in apoptotic execution. Survivin is a member of inhibitors of
apoptosis and ursodeoxycholic acid (UDCA) prevents apoptosis from various
apoptotic stimuli. To assess the activity of survivin and the effect of UDCA
on the survivin in ER stress-mediated apoptosis, we treated hepatoma cell
lines with thapsigargin (TG). TG-induced apoptosis was assessed by
morphological changes, DNA fragmentation, cleavages of
poly(ADP-ribose)polymerase (PARP), and activation of calpain and caspase-12.
The level of survivin was decreased after TG treatment in hepatoma cell lines
indicating that survivin play an important role in ER stress-mediated
apoptosis. UDCA prevented decrease in survivin levels and inhibited TG-induced
apoptosis and caspase-12 activation suggesting an anti-apoptotic effect of
UDCA.
PMID: 12609713 [PubMed - indexed for MEDLINE]
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